Journal: Inflammatory bowel diseases

Article Title: Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.

doi: 10.1097/MIB.0000000000000105

Figure Lengend Snippet: FIGURE 3. A, IL-23 secretion of sorted ILCs populations cocultured with or without macrophages, as determined by enzyme-linked immunosorbent assay (ELISA), n ¼ 3. B, The open bars show IL-22 secretion of sorted cells cocultured with macrophages under LPS stimulation measured by ELISA. The filled bars show sorted cells co- cultured with macrophages and stimulated with LPS and anti-IL-23 antibody (10 mg/mL), n ¼ 3. C, IL-23 receptor messenger RNA was quantified and normalized to 18S RNA expression, n ¼ 12. *P , 0.05.

Article Snippet: The concentration of IL-22 in cell culture supernatants of patients was assayed using a human IL-22 Quantikine enzymelinked immunosorbent assay kit (R&D Systems) with a detection limit of 15.6 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, RNA Expression

Journal: Inflammatory bowel diseases

Article Title: Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.

doi: 10.1097/MIB.0000000000000105

Figure Lengend Snippet: FIGURE 5. A, The percentage of each population from inflamed lesions (n ¼ 23) and noninflamed lesions (n ¼ 20). B, The open bars show IL-22 secretion levels of ILCs cocultured with macrophages from inflamed lesions. The filled bars show ILCs cocultured from noninflamed lesions in the same individuals. The secretion levels were measured by enzyme-linked immunosorbent assay, n ¼ 4. C, The open bars show IL-23 receptor expression of ILCs from inflamed lesions. The filled bars show IL-23 receptor expression of ILCs from noninflamed lesions. IL-23 receptor mes- senger RNA was quantified and normalized to 18S RNA expression, n ¼ 5. *P , 0.05. NS, not significant.

Article Snippet: The concentration of IL-22 in cell culture supernatants of patients was assayed using a human IL-22 Quantikine enzymelinked immunosorbent assay kit (R&D Systems) with a detection limit of 15.6 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, RNA Expression

Journal: BMC Cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Summary of tissue expression of Sp17 by type of epithelium or neoplasm

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: Expressing, Staining

Journal: BMC Cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Representative Sp17 immunohistochemistry staining in ovarian tissue of serous histology. Examples of Sp17 protein expression in benign and malignant ovarian tissue demonstrating both positive and negative staining and intensity of Sp17 staining are shown. a ) Serous cystadenoma showing tissue staining positive with strong expression of Sp17 in ciliated cells. b ) Serous borderline tumor showing strong Sp17 expression. c ) Grade 1 serous adenocarcinoma negative for Sp17 expression. d ) Grade 1 serous adenocarcinoma showing strong Sp17 expression. e ) Grade 3 serous adenocarcinoma negative for Sp17. f ) Grade 3 serous adenocarcinoma demonstrating strong Sp17 staining. Stroma surrounding epithelial cells is negative

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: Immunohistochemistry, Staining, Expressing, Negative Staining

Journal: BMC Cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Univariate and multivariable analysis of Sp17 tissue expression among epithelial ovarian carcinoma specimens

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: Expressing, Biomarker Discovery

Journal: BMC Cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Sp17 RNA expression is upregulated in borderline ovarian cancers. Sp17 mRNA expression was analyzed from two publically available patient tumor Oncomine datasets. a Sp17 mRNA levels are significantly increased in borderline ovarian serous adenocarcinoma when compared to ovarian serous adenocarcinoma ( p < 0.001) in Anglesio et al. dataset . b Sp17 mRNA levels are significantly increased in borderline ovarian serous adenocarcinoma when compared to ovarian adenocarcinoma (p < 0.001) in Tothill et al. dataset ( c ) No significant difference in Sp17 expression is seen between grades of ovarian adenocarcinoma ( p = 0.42) . Box encompasses 25th–75th percentile, solid black line indicates median and error bars indicate range. Numbers in parentheses indicate number of cases included in the analysis

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: RNA Expression, Expressing

Journal: BMC Cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Median Sp17 serum concentration by type of ovarian neoplasm

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: Concentration Assay

Journal: BMC Cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Serum Sp17 concentration compared to histology and tissue expression. a Distribution of serum Sp17 concentration in benign ovarian neoplasms, borderline ovarian tumors (BOTs), and epithelial ovarian carcinomas (EOCs). The serum concentration of Sp17 is plotted in standard box and whisker plots on a log 10 scale by histology. No significant difference was found between the serum Sp17 concentration when comparing benign ovarian neoplasms, BOTs, and EOCs. ANOVA test showed that there was a significant difference (p < 0.001) in serum Sp17 concentration among the four main histologic subtypes of EOC: serous, mucinous, endometrioid, and clear cell. Box (interquartile range) encompasses 25th–75th percentile, solid black line indicates median and the whiskers indicate the lowers and highest data values that are still within the 25th and 75th percentile value plus 1.5 times the interquartile range, respectively. The outliers are represented as separate data points. Numbers in parentheses indicate number of cases included in the analysis. b Distribution of serum Sp17 concentration by tissue expression of Sp17. There were 65 patients with both serum and ovarian tissue available, which included benign, borderline, and malignant ovarian neoplasms. The ovarian tissue underwent immunohistochemical (IHC) staining for Sp17 and any expression was considered a positive result. The matching sera for these patients were analyzed for Sp17 concentration using ELISA and the concentration converted to a log 10 scale and represented here in box and whisker plots. There was a significant difference in serum Sp17 levels between IHC positive neoplasms compared to IHC negative neoplasms ( p = 0.027), with IHC positive neoplasms showing higher SP17 levels. Box (interquartile range) encompasses 25th–75th percentile, solid black line indicates median and the whiskers indicate the range. Numbers in parentheses indicate number of cases included in the analysis

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: Concentration Assay, Expressing, Whisker Assay, Immunohistochemical staining, Immunohistochemistry, Enzyme-linked Immunosorbent Assay

Journal: International immunology

Article Title: IL-17 and IL-22 enhance skin inflammation by stimulating the secretion of IL-1β by keratinocytes via the ROS-NLRP3-caspase-1 pathway.

doi: 10.1093/intimm/dxr110

Figure Lengend Snippet: Fig. 1. IL-17 and IL-22 receptors are expressed in HaCaT cells. (A) Cultured HaCaT cells were analyzed by RT–PCR to confirm the constitutive expression of IL-17RA (167 bp) and IL-22R (183 bp). (B) Surface expression of IL-17RA and IL-22R (open area) on HaCaT single-cell suspensions compared with those of the isotype controls (filled area) by flow cytometry.

Article Snippet: To block IL-22R in HaCaT cells, the cells were incubated with either rabbit anti-human IL-22R antibody (AF2770; R&D systems) or with the isotype control (AB-108-C; R&D systems) at a concentration of 50 lg ml 1 for 2 h prior to the addition of IL-22 to the cell culture. siRNA silencing of NLRP3 expression To reduce endogenous NLRP3 expression, HaCaT cells were transfected with 80 pmol of siRNA oligonucleotides (sc-45469; Santa Cruz Biotechnology) in accordance with the manufacturer’s instructions.

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Expressing, Cytometry

Journal: International immunology

Article Title: IL-17 and IL-22 enhance skin inflammation by stimulating the secretion of IL-1β by keratinocytes via the ROS-NLRP3-caspase-1 pathway.

doi: 10.1093/intimm/dxr110

Figure Lengend Snippet: Fig. 5. Anti-IL-22R Ab-treated HaCaT cells down-regulate IL-1b production and result in reduced expression of the NLRP3-mediated caspase-1 activation pathway. To block IL-22R-mediated signaling, pre-treatment of HaCaT cells with 50 lg ml1 of anti-IL-22R blocking antibody for 2 h prior to IL-22 stimulation resulted in reduced NLRP3 and caspase-1 expression (A) as well as a consequent reduction in IL-1b secretion as measured by ELISA (B). The data are expressed as the means 6 SEMs (*P < 0.05). Ab, anti-IL-22R blocking antibody; IgG, isotype antibody for the negative control of IL-22R antibody.

Article Snippet: To block IL-22R in HaCaT cells, the cells were incubated with either rabbit anti-human IL-22R antibody (AF2770; R&D systems) or with the isotype control (AB-108-C; R&D systems) at a concentration of 50 lg ml 1 for 2 h prior to the addition of IL-22 to the cell culture. siRNA silencing of NLRP3 expression To reduce endogenous NLRP3 expression, HaCaT cells were transfected with 80 pmol of siRNA oligonucleotides (sc-45469; Santa Cruz Biotechnology) in accordance with the manufacturer’s instructions.

Techniques: Expressing, Activation Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: American Journal of Translational Research

Article Title: P27 deletion enhances hematopoiesis by paracrine action of IL22 secreted from bone marrow mesenchymal stem cells

doi:

Figure Lengend Snippet: Sequences of primers employed for Real time RT-PCR

Article Snippet: IL22 condition culture medium included normal medium (hereinafter reported as NM, consist of 100% basic culture medium) with different concentration (0 μg/L, 50 μg/L and 100 μg/L) of recombinant mouse IL22 (R & D Systems, Inc., USA) and KO-CM with different concentration (0 μg/ml, 1 μg/ml, 2 μg/ml and 4 μg/ml) of polyclonal rabbit anti-IL22 antibody (Millipore, USA).

Techniques: Sequencing

Journal: American Journal of Translational Research

Article Title: P27 deletion enhances hematopoiesis by paracrine action of IL22 secreted from bone marrow mesenchymal stem cells

doi:

Figure Lengend Snippet: Identify paracrine factors released from p27 deficient BM-MSCs. (A) Representative graphs of protein chip assays for the WT-CM and KO-CM. (B) List of up- or down-regulated proteins in KO-CM relative to WT-CM over 2 times. (C) IL22 mRNA related expression levels in WT and KO BM-MSCs. (D) Representative Western blots for IL22 and p27 protein expression, and (E) IL22 protein related expression levels in WT and KO BM-MSCs. **P < 0.01, compared with WT BM-MSCs.

Article Snippet: IL22 condition culture medium included normal medium (hereinafter reported as NM, consist of 100% basic culture medium) with different concentration (0 μg/L, 50 μg/L and 100 μg/L) of recombinant mouse IL22 (R & D Systems, Inc., USA) and KO-CM with different concentration (0 μg/ml, 1 μg/ml, 2 μg/ml and 4 μg/ml) of polyclonal rabbit anti-IL22 antibody (Millipore, USA).

Techniques: Expressing, Western Blot

Journal: American Journal of Translational Research

Article Title: P27 deletion enhances hematopoiesis by paracrine action of IL22 secreted from bone marrow mesenchymal stem cells

doi:

Figure Lengend Snippet: IL22 mediated p27 deletion-induced HSC/HPC expansion. The percentages of (A) HSCs and (B) HPCs in WT BMC cultures with 0, 50 or 100 μg/L IL22. *P < 0.05, compared with control cultures. The percentages of (C) HSCs and (D) HPCs in WT BMC cultures with KO-CM containing 0, 1, 2, 3, 4 μg/ml anti-IL22 antibody. *P < 0.05, **P < 0.01, ***P < 0.001, compared with control cultures only with KO-CM. (E) Representative images for CFCs from WT BMC cultures with NM or IL22 or KO-CM or KO-CM+IL22 antibody. (F) The number of CFCs. *P < 0.05, **P < 0.01, compared with NM control cultures. #P < 0.05, ###P < 0.001, compared with KO-CM cultures.

Article Snippet: IL22 condition culture medium included normal medium (hereinafter reported as NM, consist of 100% basic culture medium) with different concentration (0 μg/L, 50 μg/L and 100 μg/L) of recombinant mouse IL22 (R & D Systems, Inc., USA) and KO-CM with different concentration (0 μg/ml, 1 μg/ml, 2 μg/ml and 4 μg/ml) of polyclonal rabbit anti-IL22 antibody (Millipore, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: P27 deletion enhances hematopoiesis by paracrine action of IL22 secreted from bone marrow mesenchymal stem cells

doi:

Figure Lengend Snippet: IL22-Stat3 signaling mediated p27 deletion-induced HSC/HPC expansion. The percentages of IL22RA1 in (A) HSCs and (B) HPCs in BMCs from WT and p27 KO mice. The percentages of Stat3 in (C) HSCs and (D) HPCs in BMCs from WT and p27 KO mice. The percentages of p-Stat3 (S727) in (E) HSCs and (F) HPCs in BMCs from WT and p27 KO mice. *P < 0.05, compared with WT mice.

Article Snippet: IL22 condition culture medium included normal medium (hereinafter reported as NM, consist of 100% basic culture medium) with different concentration (0 μg/L, 50 μg/L and 100 μg/L) of recombinant mouse IL22 (R & D Systems, Inc., USA) and KO-CM with different concentration (0 μg/ml, 1 μg/ml, 2 μg/ml and 4 μg/ml) of polyclonal rabbit anti-IL22 antibody (Millipore, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: P27 deletion enhances hematopoiesis by paracrine action of IL22 secreted from bone marrow mesenchymal stem cells

doi:

Figure Lengend Snippet: Mechanism of p27 deletion in enhancing hematopoiesis. p27 deficiency stimulates HSC/HPC expansion by increasing secretion of IL22 by BM-MSCs and activating IL22-Stat3 signaling in HSCs and HPCs.

Article Snippet: IL22 condition culture medium included normal medium (hereinafter reported as NM, consist of 100% basic culture medium) with different concentration (0 μg/L, 50 μg/L and 100 μg/L) of recombinant mouse IL22 (R & D Systems, Inc., USA) and KO-CM with different concentration (0 μg/ml, 1 μg/ml, 2 μg/ml and 4 μg/ml) of polyclonal rabbit anti-IL22 antibody (Millipore, USA).

Techniques:

Journal: Molecular Medicine

Article Title: Paris saponin VII attenuates psoriasiform inflammation by regulating STAT3/NFκB signaling pathway and Caspase-1-induced pyroptosis

doi: 10.1186/s10020-025-01253-y

Figure Lengend Snippet: PSVII exhibited anti-inflammatory and antioxidant effects in IMQ-induced psoriasis-like mice. ( A ) ELISA analysis of serum levels of IL-17, IL-23, IL-2, IL-6 and TNF-α. ( B ) and ( C ) Relative expression of TNF-α in PSVII -treated psoriasis models in vitro. ( D ) Immunofluorescence microscopy of HaCaT sections treated with PSVII , probing for TNF-α infiltration (scale bar = 10 μm). ( E ) ROS expression in various groups, as observed under fluorescence microscopy (scale bar = 20 μm). n = 6 mice/group, n = 3 cells/group. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Model group

Article Snippet: The cells were divided into the following groups: (Griffiths et al. ) Control group; (Chandran ) Model group; and (Lee and Kim ) PSVII treatment groups, which received PSVII at concentrations of 2 μM, 5 μM, and 10 μM (designated as PSVII -L, PSVII -M, and PSVII -H, respectively), for 24 h. A psoriasis-like model was induced in the model group using M5 (The cytokine mixture composed of IL-17, IL-22, IL-1α, TNF-α, and Oncostatin M, the concentration was 10 ng/ml, Novoprotein, China), as previously described (Zhao et al. ).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, In Vitro, Immunofluorescence, Microscopy, Fluorescence